EPISODE #31 - HOW TO SET PMT VOLTAGES Determining the optimal voltage to set the detector to is an important step in ensuring that fluorescence signal can be properly measured. It is important to start with an optimized PMT. Since beads do not necessarily have the same fluorochromes as are being used in the experiment, a voltration experiment is in order to optimize the experimental specific voltage. This is performed using single-stained cells, that have been stained with properly titrated anti